Search | Engineering

订阅投稿

首页工程期刊工程焦点工程成就工程前沿关于我们 English

资源类型

期刊论文 663

年份

2024 3

2023 76

2022 77

2021 75

2020 74

2019 54

2018 52

2017 47

2016 40

2015 43

2014 34

2013 10

2012 18

2011 7

2010 7

2009 5

2008 10

2007 5

2006 3

2005 2

展开︾

关键词

医院中子照射器I型堆 6

5G 2

COVID-19 2

信息技术 2

免疫球蛋白G 2

状态监测 2

肠道菌群 2

&alpha 1

(GaxIn1−x)2O3薄膜；带隙可调谐；磁控溅射 1

1 1

2-羟基丁酸 1

2016年熊本地震 1

5% 法 1

5-戊二胺 1

5G 技术 1

5G；IMT-2020；关键技术；标准；现场试验 1

5G；交叉振子；双极化天线；终端天线；超宽带 1

5型腺病毒 1

展开︾

检索范围：

排序：展示方式：

碳青霉烯类耐药性在人源和动物源产NDM酶大肠埃希菌间的传播 Article

沈应博, 胡付品, 王永强, 尹丹丹, 杨璐, 陈义强, 许春燕, 李基云, 蒋君瑶, 王雪杨, 付玉林, 邵东延, 刘德俊, 马藤菲, 蔡畅, 沈张奇, 王少林, 李娟, 张嵘, 柯跃斌, 吴聪明, 沈建忠, Timothy R. Walsh, 汪洋

《工程（英文）》 2022年第15卷第8期页码 24-33 doi: 10.1016/j.eng.2021.07.030

摘要：

Although carbapenem use is prohibited in animals in China, carbapenem-resistant Escherichia coli (CREC), especially New Delhi metallo-β-lactamase (NDM)-producing strains, are widely prevalent in foodproducing animals. At present, the impact of livestock-associated CREC strains on human populations at the national level is unknown. Here, we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence. We also ascertain the bla_NDM and bla_KPC abundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin. Overall, 631/29799 (2.1%) clinical Escherichia coli (E. coli) isolates were identified as CREC. Multivariable analysis revealed that being male, an age below 1, an age between 13 and 18, provinces with greater chicken production, and provinces with higher pig production were associated with higher odds of CREC infection. In general, 73.8% (n = 45/61) of pig farms and 62.2% (n = 28/45) of chicken farms had a bla_NDM abundance of 1×10^－5 to 1×10^－3 and 1×10－3 to 1×10^－2, respectively. Among all the Chinese NDM-positive E. coli (n = 463) available at the National Center for Biotechnology Information (NCBI), the genomic analysis revealed that bla_NDM-5 and IncX3 were the predominant carbapenemase gene-plasmid combination, while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels. All the findings suggest frequent CREC transmission between humans and animals, indicating that further discussions on the use of antibiotics in animals and humans are needed, both in China and across the globe.

关键词： Carbapenem resistance Escherichia coli bla^NDM-5 One Health Antimicrobial resistance transmission

HTML PDF 收藏

K+sup> and Na+sup> fluxes in roots of two Chinese Iris populations

Pinfang LI,Biao ZHANG

《农业科学与工程前沿（英文）》 2014年第1卷第2期页码 144-149 doi: 10.15302/J-FASE-2014016

摘要： Maintenance of ion homeostasis, particularly the regulation of K and Na uptake, is important for all plants to adapt to salinity. Observations on ionic response to salinity and net fluxes of K , Na in the root exhibited by plants during salt stress have highlighted the need for further investigation. The objectives of this study were to compare salt adaptation of two Chinese Iris ( Pall. var. (Fisch.) Koidz.) populations, and to improve understanding of adaptation to salinity exhibited by plants. Plants used in this study were grown from seeds collected in the Xinjiang Uygur Autonomous Region (Xj) and Beijing Municipality (Bj), China. Hydroponically-grown seedlings of the two populations were supplied with nutrient solutions containing 0.1 (control) and 140 mmol·L NaCl. After 12 days, plants were harvested for determination of relative growth rate and K , Na concentrations. Net fluxes of K , Na from the apex and along the root axis to 10.8 mm were measured using non-invasive micro-test technique. With 140 mmol·L NaCl treatment, shoots for population Xj had larger relative growth rate and higher K concentration than shoots for population Bj. However, the Na concentrations in both shoots and roots were lower for Xj than those for Bj. There was a lower net efflux of K found in population Xj than by Bj in the mature zone (approximately 2.4-10.8 mm from root tip). However, no difference in the efflux of Na between the populations was obtained. Population Xj of continued to grow normally under NaCl stress, and maintained a higher K /Na ratio in the shoots. These traits, which were associated with lower K leakage, help population Xj adapt to saline environments.

关键词： Iris lactea Pall. var. chinensis (Fisch.) Koidz population K⁺ and Na⁺ ion flux non-invasive micro-test technique

HTML PDF 收藏

High frequency of alternative splicing variants of the oncogene in neuroendocrine tumors of the pancreas and breast

《医学前沿（英文）》页码 907-923 doi: 10.1007/s11684-023-1009-7

摘要： The characteristic genetic abnormality of neuroendocrine neoplasms (NENs), a heterogeneous group of tumors found in various organs, remains to be identified. Here, based on the analysis of the splicing variants of an oncogene Focal Adhesion Kinase (FAK) in The Cancer Genome Atlas datasets that contain 9193 patients of 33 cancer subtypes, we found that Box 6/Box 7-containing FAK variants (FAK^6/7) were observed in 7 (87.5%) of 8 pancreatic neuroendocrine carcinomas and 20 (11.76%) of 170 pancreatic ductal adenocarcinomas (PDACs). We tested FAK variants in 157 tumor samples collected from Chinese patients with pancreatic tumors, and found that FAK^6/7 was positive in 34 (75.6%) of 45 pancreatic NENs, 19 (47.5%) of 40 pancreatic solid pseudopapillary neoplasms, and 2 (2.9%) of 69 PDACs. We further tested FAK splicing variants in breast neuroendocrine carcinoma (BrNECs), and found that FAK^6/7 was positive in 14 (93.3%) of 15 BrNECs but 0 in 23 non-NEC breast cancers. We explored the underlying mechanisms and found that a splicing factor serine/arginine repetitive matrix protein 4 (SRRM4) was overexpressed in FAK^6/7-positive pancreatic tumors and breast tumors, which promoted the formation of FAK^6/7 in cells. These results suggested that FAK^6/7 could be a biomarker of NENs and represent a potential therapeutic target for these orphan diseases.

关键词： FAK^6/7 SRRM4 neuroendocrine neoplasms pancreas breast

HTML PDF 收藏

PtHAK5, a candidate for mediating high-affinity K

Haili YANG, Weidan ZHANG, Weiwei CHAI, Wenying WANG, Li GAO, Jing ZHANG, Yongping WANG, Suo-Min WANG

《农业科学与工程前沿（英文）》 2018年第5卷第1期页码 108-117 doi: 10.15302/J-FASE-2018200

摘要： is a typical salt-exclu-ding halophytic grass with strong salt-tolerance, which enhances tolerance by restricting Na influx as well as having a strong selectivity for K over Na . The HAK5 K transporters generally modulate effective K acquisition in plants, especially under low K condition. In this study, from was isolated by RT-PCR and characterized using yeast complementation. The results showed PtHAK5 consisted of 784 amino acids and shared over 80% homology with the identified high-affinity K transporter HAK5 from other higher plants. The expression of rescued the K -uptake-defective phenotype of yeast strain CY162. In conclusion, PtHAK5 is a candidate for mediating high-affinity K uptake under low K conditions.

关键词： K⁺ uptake PtHAK5 Puccinellia tenuiflora yeast complementation

HTML PDF 收藏

五唑阴离子——前行中的新一代高含能材料

林秋汉, 王鹏程, 许元刚, 陆明

《工程（英文）》 2020年第6卷第9期页码 964-966 doi: 10.1016/j.eng.2020.04.011

HTML PDF 收藏

♮-convexity and its applications in operations

Xin CHEN

《工程管理前沿（英文）》 2017年第4卷第3期页码 283-294 doi: 10.15302/J-FEM-2017057

摘要： -convexity, one of the central concepts in discrete convex analysis, receives significant attentions in the operations literature in recent years as it provides a powerful tool to derive structures of optimal policies and allows for efficient computational procedures. In this paper, we present a survey of key properties of -convexity and some closely related results in lattice programming, several of which were developed recently and motivated by operations applications. As a new contribution to the literature, we establish the relationship between a notion called -differential monotonicity and -convexity. We then illustrate the techniques of applying -convexity through a detailed analysis of a perishable inventory model and a joint inventory and transshipment control model with random capacities.

关键词： L^&natur -convexity lattice programming perishable inventory models random capacity

HTML PDF 收藏

On the monolayer dispersion behavior of Co₃O₄ on HZSM-5 support: designing applicable

《化学科学与工程前沿（英文）》 2023年第17卷第11期页码 1741-1754 doi: 10.1007/s11705-023-2332-x

摘要： Based on monolayer dispersion theory, Co₃O₄/ZSM-5 catalysts with different loadings have been prepared for selective catalytic reduction of nitrogen oxides by ammonia. Co₃O₄ can spontaneously disperse on HZSM-5 support with a monolayer dispersion threshold of 0.061 mmol 100 m^–2, equaling to a weight percentage around 4.5%. It has been revealed that the quantities of surface active oxygen (O₂^–) and acid sites are crucial for the reaction, which can adsorb and activate NO_x and NH₃ reactants effectively. Below the monolayer dispersion threshold, Co₃O₄ is finely dispersed as sub-monolayers or monolayers and in an amorphous state, which is favorable to generate the two kinds of active sites, hence promoting the performance of ammonia selective catalytic reduction of nitrogen oxide. However, the formation of crystalline Co₃O₄ above the capacity is harmful to the reaction performance. 4% Co₃O₄/ZSM-5, the catalyst close to the monolayer dispersion capacity, possesses the most abundant active O₂^– species and acidic sites, thereby demonstrating the best reaction performance in all the samples. It is proposed the optimal Co₃O₄/ZSM-5 catalyst can be prepared by loading the capacity amount of Co₃O₄ onto HZSM-5 support.

关键词： Co₃O₄/ZSM-5 NO_x-SCR by NH₃ monolayer dispersion threshold effect surface acid sites surface active O₂^– anions

HTML PDF 收藏

131sup>I标记的人源化抗B7-H3抗体用于胶质母细胞瘤放射免疫疗法的治疗特性 Article

傅丰庆, Meng Zheng, Shandong Zhao, Yan Wang, Minzhou Huang, Hanqing Chen, Ziyi Huang, Kaijie Zhang, 缪丽燕, 张学光

《工程（英文）》 2023年第30卷第11期页码 190-202 doi: 10.1016/j.eng.2023.05.011

摘要：

B7 homolog 3 (B7-H3) has attracted much attention in glioblastoma (GBM) radioimmunotherapy (RIT) due to its abnormally high expression on tumor cells. In this study, we report that two specific humanized anti-human B7-H3 antibodies (hu4G4 and hu4H12) derived from mouse anti-human B7-H3 antibodies that were generated by computer-aided design and exclusively recognize membrane expression of B7-H3 by human glioma cells. Hu4G4 and hu4H12 were radiolabeled with ⁸⁹Zr for RIT antibody screening. Micro-positron emission tomography (PET) imaging, biodistribution and pharmacokinetic (PK) analyses of ⁸⁹Zr-labeled antibodies were performed in U87-xenografted models. ¹²⁵I labelling of the antibodies for single-photon emission computed tomography (SPECT) imaging was also used to investigate the biological behavior of the antibodies in vivo. Furthermore, the pharmacodynamic (PD) of the 131Ilabeled antibodies were evaluated in U87-xenografted mice and GL261 Red-FLuc-B7-H3 in situ glioma tumor models. Micro-PET imaging and biodistribution analysis with a gamma counter showed that ⁸⁹Zr-deferoxamine (DFO)-hu4G4 had higher tumor targeting performance with lower liver uptake than ⁸⁹Zr-DFO (hu4H12, immunoglobulin G (IgG)). The biodistribution results of ¹²⁵I-SPECT imaging were similar to those of ⁸⁹Zr-PET imaging, though the biodistribution in long bone joints and the thyroid varied. The PD analysis results indicated that 131I-hu4G4 had an excellent therapeutic effect and high safety with no apparent toxicity. Interestingly, 131I-hu4G4 improved the tumor vasculature in tissues with higher expression of collagen type IV and platelet-derived growth factor receptor b (PDGFR-b) compared with control treatment, as determined by immunofluorescence (IF), which contributed to inhibiting tumor growth. Taken together, our data indicate that hu4G4 exhibits good tumor targeting and specificity, achieves low nonspecific concentrations in normal tissues, and has acceptable PK characteristics. 131I-hu4G4 also exerts effective antitumor effects with an ideal safety profile. Therefore, we expect hu4G4 to be an excellent antibody for the development of GBM RIT.

关键词： B7-H3 Radioimmunotherapy Glioblastoma Pharmacokinetics Pharmacodynamics

HTML PDF 收藏

Effects of ancillary ligands in acceptorless benzyl alcohol dehydrogenation mediated by phosphine-free cobalt complexes

《化学科学与工程前沿（英文）》 2023年第17卷第3期页码 314-325 doi: 10.1007/s11705-022-2219-2

摘要： Acceptorless alcohol dehydrogenation stands out as one of the most promising strategies in hydrogen storage technologies. Among various catalytic systems for this reaction, cost-effective molecular catalysts using phosphine-free ligands have gained considerable attention. However, the central challenge for using non-precious metals is to overcome the propensity of reacting by one-electron pathway. Herein, we synthesized a phosphine-free η⁵-C₅Me₅-Co complex by using the metal–ligand cooperative strategy and compared its activity with analogous catalysts toward acceptorless alcohol dehydrogenation. The catalyst showed excellent performance with a turnover number of 130.4 and a selectivity close to 100%. The improved performance among the class of η⁵-C₅Me₅-Co complexes could be attributed to the more accessible Co center and its cooperation with the redox-active ligand. To further study the systematic structure-activity relationship, we investigated the electronic structures of η⁵-C₅Me₅-Co complexes by a set of characterizations. The results showed that the redox-active ligand has a significant influence on the η⁵-C₅Me₅-Co moiety. In the meantime, the proximal O⁻/OH group is beneficial for shuttling protons. For the catalytic cycle, two dehydrogenation scenarios were interrogated through density functional theory, and the result suggested that the outer-sphere pathway was preferred. The formation of a dihydrogen complex was the rate-determining step with a ΔG value of 16.9 kcal∙mol^‒1. The electron population demonstrated that the η⁵-C₅Me₅ ligand played a key role in stabilizing transition states during dehydrogenation steps. This work identified the roles of vital ligand components to boost catalytic performance and offered rationales for designing metal–ligand cooperative nonprecious metal complexes.

关键词： acceptorless alcohol dehydrogenation η⁵-C₅Me₅-Co metal–ligand cooperation theoretical calculation

HTML PDF 收藏

动态二叉树表示环境的A*sup>算法及其在足球机器人路径规划中的实现

唐平,杨宜民

《中国工程科学》 2002年第4卷第9期页码 50-53

摘要：

提出采用二叉树表示二维空间的方法，对全局路径规划和局部路径规划进行综合考虑，设计移动机器人在复杂环境下对动态障碍物进行避障的A*sup>算法。

关键词：动态二叉树 A*sup>算法路径规划

HTML PDF 收藏

Clinical significance of CD34+sup>CD117dimsup>/CD34+sup>CD117brisup> myeloblast-associated

Xueping Li, Yuting Dai, Bing Chen, Jinyan Huang, Saijuan Chen, Lu Jiang

《医学前沿（英文）》 2021年第15卷第4期页码 608-620 doi: 10.1007/s11684-021-0836-7

摘要： t(8;21)(q22;q22) acute myeloid leukemia (AML) is a highly heterogeneous hematological malignancy with a high relapse rate in China. Two leukemic myeloblast populations (CD34 CD117 and CD34 CD117 ) were previously identified in t(8;21) AML, and CD34 CD117 cell proportion was determined as an independent factor for this disease outcome. Here, we examined the impact of CD34 CD117 /CD34 CD117 myeloblast-associated gene expression on t(8;21) AML clinical prognosis. In this study, 85 patients with t(8;21) AML were enrolled. The mRNA expression levels of CD34 CD117 -associated genes ( , , and ) and CD34 CD117 -associated genes ( , , and ) were measured using quantitative reverse transcription PCR. Associations between gene expression and clinical outcomes were determined using Cox regression models. Results showed that patients with high , , or expression had significantly inferior overall survival (OS), whereas those with high or expression showed relatively favorable prognosis. Univariate analysis revealed that CD19, CD34 CD117 proportion, mutation, minimal residual disease (MRD), and expression levels of , , , and were associated with OS. Multivariate analysis indicated that mutation, MRD and and expression levels were independent prognostic variables for OS. Identifying the clinical relevance of CD34 CD117 /CD34 CD117 myeloblast-associated gene expression may provide new clinically prognostic markers for t(8;21) AML.

关键词： t(8 21)(q22 q22) AML CD34⁺CD117^dim/ CD34⁺CD117^bri cell population gene expression prognosis

HTML PDF 收藏

Removal of ammonium and nitrate through Anammox and FeS-driven autotrophic denitrification

《环境科学与工程前沿（英文）》 2023年第17卷第6期 doi: 10.1007/s11783-023-1674-4

摘要：

● Simultaneous NH₄⁺/NO₃^– removal was achieved in the FeS denitrification system

关键词： Anammox Denitrification FeS NH₄⁺/NO₃⁻ Sulfammox

HTML PDF 收藏

Characterization of NDM-1-producing carbapenemase in

Rongmin ZHANG,Yang WANG,Zhihai LIU,Jiyun LI,Wenjuan YIN,Lei LEI,Congming WU,Jianzhong SHEN

《农业科学与工程前沿（英文）》 2015年第2卷第3期页码 223-229 doi: 10.15302/J-FASE-2015065

摘要： In recent years, the mobile metallo-β-lactamase (MBL) genes have been found to correspond to one of the most important resistance characters identified in Gram-negative bacteria, severely affecting clinical chemotherapy and threatening public health. The prevalence of mobile MBL genes and their flanking regions in Gram-negative bacteria from diseased pigs in China was investigated. A total of 334 lung samples from diseased pigs were screened for Gram-negative bacteria classified as non-susceptible to meropenem (MIC≥4 mg·L ). Six isolates, including three , two and one , exhibited MBL production and carried the gene. S1-PFGE and Southern blot analysis showed that the gene was located on the chromosome of one isolate and on plasmids of various sizes in the other five isolates. MIC testing using broth microdilution revealed that all -carrying isolates and some of their transconjugants exhibited resistance to almost all β-lactams tested. Whole genome sequencing revealed that the flanking region of the gene from all porcine isolates had high levels of similarity with the corresponding regions in human isolates. One porcine isolate carrying was typed as ST48, a common sequence type in human isolates. These results suggest the possibility of human-to-food animal transfer of -producing , highlighting the need for surveillance of carbapenemase producers among bacteria from food animals. In addition, the prudent use of antimicrobial agents to decrease the opportunities for co-selection of carbapenemase genes in food animals is also urgently needed.

关键词： carbapenemase NDM-1 ISAba125 Enterobacteriaceae food safety

HTML PDF 收藏

Selective recovery of Cu

Haiping LUO,Bangyu QIN,Guangli LIU,Renduo ZHANG,Yabo TANG,Yanping HOU

《环境科学与工程前沿（英文）》 2015年第9卷第3期页码 522-527 doi: 10.1007/s11783-014-0633-5

摘要： As the bioelectrochemical system, the microbial fuel cell (MFC) and the microbial electrolysis cell (MEC) were developed to selectively recover Cu and Ni ions from wastewater. The wastewater was treated in the cathode chambers of the system, in which Cu and Ni ions were removed by using the MFC and the MEC, respectively. At an initial Cu concentration of 500 mg·L , removal efficiencies of Cu increased from 97.0%±1.8% to 99.0%±0.3% with the initial Ni concentrations from 250 to 1000 mg·L , and maximum power densities increased from 3.1±0.5 to 5.4±0.6 W·m . The Ni removal mass in the MEC increased from 6.8±0.2 to 20.5±1.5 mg with the increase of Ni concentrations. At an initial Ni concentration of 500 mg·L , Cu removal efficiencies decreased from 99.1%±0.3% to 74.2%±3.8% with the initial Cu concentrations from 250 to 1000 mg·L , and maximum power densities increased from 3.0±0.1 to 6.3±1.2 W·m . Subsequently, the Ni removal efficiencies decreased from 96.9%±3.1% to 73.3%±5.4%. The results clearly demonstrated the feasibility of selective recovery of Cu and Ni from the wastewater using the bioelectrochemical system.

关键词： bioelectrochemical system Cu²⁺ Ni²⁺ selective recovery